Semester 7
BP701T. INSTRUMENTAL METHODS OF ANALYSIS (Theory)
Scope: This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart fundamental knowledge on the principles and instrumentation of spectroscopic and chromatographic techniques. This also emphasizes theoretical and practical knowledge of modern analytical instruments that are used for drug testing.
Objectives: Upon completion of the course the student shall be able to
1. Understand the interaction of matter with electromagnetic radiation and its applications in drug analysis
2. Understand the chromatographic separation and analysis of drugs.
3. Perform quantitative & qualitative analysis of drugs using various analytical instruments.
Course Content:
UNIT –I
UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.
Applications - Spectrophotometric titrations, Single-component and multi-component analysis
Fluorimetry
Theory, Concepts of singlet, doublet, and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation, and applications
UNIT –II
IR spectroscopy
Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations
Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications
Flame Photometry-Principle, interferences, instrumentation and applications
Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications
Nepheloturbidometry- Principle, instrumentation, and applications
UNIT –III
Introduction to chromatography
Adsorption and partition column chromatography-Methodology, advantages, disadvantages, and applications.
Thin-layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
Paper chromatography-Introduction, methodology, development techniques, advantages, disadvantages, and applications
Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications
UNIT –IV
Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications
High-performance liquid chromatography (HPLC)-Introduction, theory, instrumentation, advantages and applications.
UNIT –V
Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of the ion exchange process, factors affecting ion exchange, methodology, and applications
Gel chromatography- Introduction, theory, instrumentation, and applications
Affinity chromatography- Introduction, theory, instrumentation, and applications
Please do not enter any spam link in the comment box.